In William Shakespeare’s tragedy “Macbeth,” the theme of guilt permeates the narrative, influencing the actions and psyches of the characters, particularly Macbeth and Lady Macbeth. Guilt manifests in various forms throughout the play, from the initial pangs of conscience to overwhelming psychological torment. This essay delves into how Shakespeare explores the theme of guilt through the character arcs of Macbeth and Lady Macbeth, the symbolic use of blood and darkness, and the moral consequences that accompany their actions. By examining these elements, we gain a deeper understanding of how guilt serves as a powerful force that shapes the characters’ fates and underscores the play’s moral lessons.